We carry out research for the e-liquids of tomorrow


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happy liquid research

Since the founders of Happy People GmbH have a scientific background, it was a priority for us to incorporate science into the world of vaping from the very beginning. We try to collect research data relevant to e-cigarettes, whether in the basic research or in various special areas. happy liquid is the first e-liquid brand in the world to campaign for research in the area of e-liquids and e-cigarettes and publish their results nationally and internationally. All insights we gain on the topic of e-liquids and e-cigarettes are published here.

Publications

Innovations in Food Technology Feb. 2015: Tobacco smoke vs e-liquid vapour – Diverging toxic effects on human lung cells in culture

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Innovations in Food Technology Aug. 2015: Further in vitro studies on the cytotoxic effects of cigarette smoke vs. e-liquid vapour

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GFN 2015: Effect of cigarette smoke and e-liquid vapour on ciliary beat frequency (CBF) of freshly isolated human nasal epithelial cells

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GFN 2015: Acute and long-term cytotoxicity of cigarette smoke and e-liquid vapour on
cultured human lung cells

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GFN 2015: Effects of cigarette smoke and e-liquid vapour on mass cultures of human lung cells:
Continuous monitoring by time-lapse videography

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GFN 2016: Acute human lung cell toxicity of some selected flavouring chemicals
after simulation of vaping

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GFN 2016: Acute toxicity of a flavoured e-liquid according to TPD 2 is related to e-cigarette vaporiser resistance and electrical power

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But research strives and struggles, never tiring,
for the law, the reason, why and how.

Johann Wolfgang von Goethe (1749 - 1832)
German poet, scientist and statesman

Videos

Some videos were created as a result of our research on human lung cell cultures. These were kindly made available by Prof. Dr. Peter C. Dartsch of Dartsch Scientific GmbH.

In this case, the vapor of the tested e-liquids (as well as smoke from tobacco cigarettes) was applied in aqueous solution to cell cultures. The changes were photographed over a period of 10 hours. A time-lapse film was created from the film material from each experiment.

(Film material in time lapse created with a Basler acA 1600-20uc digital camera on an Olympus IX-50 inverted microscope)

happy liquid

 

The vapor of the “strawberry-menthol” happy liquid was added to an aqueous medium. This medium was added to a culture of human lung cells and the cell behavior was recorded in time lapse over a period of 10 hours. Here you can see no damage to the cell culture and detect normal cell divisions (mitoses), cell migration and only a few dying cells.

e-liquid from US

 

For comparison, the vapor of e-liquid from the United States was added to an aqueous medium. This medium was added to a culture of human lung cells and the cell behavior was recorded in time lapse over a period of 10 hours. In this case, you can see significant damage and cell changes. Cell divisions are no longer proceeding as they should and there is significantly decreased cell migration. The occurrence of many apoptotic and dying cells is particularly striking.

Tobacco Smoke

 

In this instance, tobacco smoke was added to an aqueous medium. This medium was added to a culture of human lung cells and the cell behavior was recorded in time lapse over a period of 10 hours. You can clearly see that the cell division and migration stops after only 4 hours and they start to die off increasingly. Finally, all cells have died off.

Do you have any questions?

Would you like to know more about our research projects or are you interested in the background? Perhaps we can answer some of your questions here.

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    Why is happy liquid the only company in Germany that actively does research on e-liquids?

    Since the very beginning, happy liquid has set very high standards; we see ourselves in a leadership role when it comes to establishing meaningful standards and guaranteeing the highest possible product safety. For this reason, we not only study the finished product, but also the individual substances as base research.

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    What does the research mean for the customers?

    Our customers can always rest assured that they are using the best product available. Now and in the future.

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    Does the happy liquid research hurt animals?

    No. We do not use animal testing to study the effects of e-liquid or vapor on the body. People use happy liquid, so we use human lung cells to examine the effects and possible side effects.